The Wallabies have been forced into a late change after Adam Coleman was ruled out with a thumb injury.
The lock failed a final fitness test on Friday morning, with 26-year old Blake Enever straight into the starting side and set to debut against England on Saturday.
The Brumbies lock will become Wallaby 916 when he runs out at Twickenham, and the Wallabies 26th debutant since the Rugby World Cup Final in 2015 at the same venue.
Michael Cheika and his coaching staff have also finalised the reserves with the Wallabies opting for a six-two split on the bench.
Lopeti Timani is the sole inclusion with Henry Speight dropping off the extended bench that was named yesterday.
Wallabies updated team to play England at Twickenham Stadium
1. Scott Sio (41 Tests)
2. Tatafu Polota-Nau (80 Tests)
3. Sekope Kepu (89 Tests)
4. Rob Simmons (80 Tests)
5. Blake Enever*
6. Ned Hanigan (11 Tests)
7. Michael Hooper (c) (77 Tests)
8. Sean McMahon (24 Tests)
9. Will Genia (vc) (86 Tests)
10. Bernard Foley (vc) (53 Tests)
11. Reece Hodge (22 Tests)
12. Samu Kerevi (16 Tests)
13. Tevita Kuridrani (56 Tests)
14. Marika Koroibete (6 Tests)
15. Kurtley Beale (69 Tests)
16. Stephen Moore (127 Tests)
17. Tom Robertson (17 Tests)
18. Allan Alaalatoa (21 Tests)
19. Matt Philip (2 Tests)
20. Ben McCalman (51 Tests)
21. Lopeti Timani (10 Tests)
22. Nick Phipps (60 Tests)
23. Karmichael Hunt (4 Tests)